An Intermediate Conformational State of Cytochrome P450cam-CN in Complex with Putidaredoxin
نویسندگان
چکیده
منابع مشابه
Detection of a high-barrier conformational change in the active site of cytochrome P450cam upon binding of putidaredoxin.
The orientation of the substrate camphor in the active site of reduced CO-bound cytochrome P450cam (CYP101) as a function of reduced putidaredoxin (Pdxr) addition has been examined by NMR using perdeuterated CYP101 and perdeuterated Pdx as well as isotopically labeled d-camphor. This permits the 1H resonances of CYP101-bound camphor to be observed without interference from the signals of CYP101...
متن کاملThe Role of Water Molecules in the Association of Cytochrome P450cam with Putidaredoxin
We have investigated the osmotic pressure dependence of the association between ferric cytochrome P450cam and putidaredoxin (Pdx) to gain an insight into the role of water molecules in the P450cam-reduced Pdx complexation amenable to physiological electron transfer. The association constant was evaluated from the electron transfer rates from reduced Pdx to P450cam. The natural logarithm of the ...
متن کاملThe role of water molecules in the association of cytochrome P450cam with putidaredoxin. An osmotic pressure study.
We have investigated the osmotic pressure dependence of the association between ferric cytochrome P450cam and putidaredoxin (Pdx) to gain an insight into the role of water molecules in the P450cam-reduced Pdx complexation amenable to physiological electron transfer. The association constant was evaluated from the electron transfer rates from reduced Pdx to P450cam. The natural logarithm of the ...
متن کاملCombined QM/MM Studies of Binding Effect of Cytochrome P450cam to Putidaredoxin
Combined QM/MM calculations of an activesite of Cytochrome P450cam have been performed, before and after a binding process of Cytochrome P450cam to Putidaredoxin. The calculations have been carried out for two coordination spheres of the heme active-site of Cytochrome P450cam, namely a 5-coordinated, and a 6coordinated system of this protein having a water molecule as a 6th ligand. An experimen...
متن کاملReduced oxy intermediate observed in D251N cytochrome P450cam.
Cytochrome P450s are ubiquitous heme proteins responsible for various oxidative metabolic processes. The overall rate-determining step in the catalytic cycle of native cytochrome P450cam is the reduction of the dioxygen complex, which has made detection of catalytic intermediates after this reduction impossible. However, for the site-specific mutant D251N cytochrome P450cam (which affects proto...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2019
ISSN: 0006-2960,1520-4995
DOI: 10.1021/acs.biochem.9b00192